May 28, 2014
leica time lapse to quicktime file
better quality in AVI files, but mac doesn't have apps to open AVI files.
The way to transfer time lapse movies generated by Leica microscope to mov files keeping the quality
1. all files can be exported as a tiff file.
2. on the mac, use ImageJ to open all images as sequence
3. save as quicktime movie using mpeg4 and max quality
The way to transfer time lapse movies generated by Leica microscope to mov files keeping the quality
1. all files can be exported as a tiff file.
2. on the mac, use ImageJ to open all images as sequence
3. save as quicktime movie using mpeg4 and max quality
April 22, 2014
Balloon disease
After the first ovulation in tropicalis, sometimes frogs get balloon desease.
treat them in 0.01g/liter Nalidixic acid (SIGMA N4382) for several days.
treat them in 0.01g/liter Nalidixic acid (SIGMA N4382) for several days.
December 18, 2013
Re-annealing PCR product for HMA
95˚C, 5 min. → 85˚C (-2 ˚C/s) → 25˚C (-0.1 ˚C/s) in annealing buffer (10Xbuffer: 1M NaCl, 100mM Tris, ph 7.8, 20mM EDTA)
I have made:-
10X annealing buffer
5M NaCl 200 µl
1M Tris-HCl (ph 7.5) 100 µl
0.5M EDTA (ph 8.0) 40 µl
water 660 µl
I have made:-
10X annealing buffer
5M NaCl 200 µl
1M Tris-HCl (ph 7.5) 100 µl
0.5M EDTA (ph 8.0) 40 µl
water 660 µl
December 17, 2013
DPI
Diphenyleneiodonium chloride (D2926, Sigma)
2 mM-10 mM stock in DMSO
For 10 mM stock, 10 mg was dissolved in 3.18 ml DMSO
Heat and vortex
store aliquots in -20˚C
Rotenone
Rotenone, R8875 Sigma
100 mM stock in DMSO can be made.
I have made 10 mM stock
0.04g/10 ml DMSO very easy
Powder can be stored in room Temp and avoid light
Stock solution can be stored in -80 ˚C for one month according to CALBIOCEHEM
100 mM stock in DMSO can be made.
I have made 10 mM stock
0.04g/10 ml DMSO very easy
Powder can be stored in room Temp and avoid light
Stock solution can be stored in -80 ˚C for one month according to CALBIOCEHEM